species-specific pcr for the diagnosis and determination of antibiotic susceptibilities of brucella strains isolated from tehran, iran
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abstract
background: brucellosis is an endemic zoonotic disease in the middle east. this study intended to design a uniplex pcr assay for the detection and differentiation of brucella at the species level and determining the antibiotic susceptibility pattern of brucella in iran. methods: sixty-eight brucella specimens (38 animal and 30 human specimens) were analyzed using pcr (using one pair of primers). antibiotic susceptibility patterns were evaluated and compared using the e-test and disk diffusion susceptibility test. tigecycline susceptibility pattern was compared with other antibiotics. results: thirty six isolates of b. melitensis, 2 isolates of b. abortus and 1 isolate of b. suis from the 38 animal specimens, 24 isolates of b. melitensis and 6 isolates of b. abortus from the 30 human specimens were differentiated. the mic50 values of doxycycline for human and animal specimens were 125 and 10 μg/ml, respectively, tigecycline 0.064 μg/ml for human specimens and 0.125μg/ml for animal specimens, and trimethoprim/ sulfamethoxazole and ciprofloxacin 0.065 and 0.125μg/ml, respectively, for both human and animal specimens. the highest mic50 value of streptomycin in the human specimens was 0.5μg/ml and 1μg/ml for the animal specimens. the greatest resistance shown was to tetracycline and gentamicin, respectively. conclusion: uniplex pcr for the detection and differentiation of brucella at the strain level is faster and less expensive than multiplex pcr, and the antibiotics doxycycline, rifampin, trimethoprim-sulfamethoxazole, ciprofloxacin, and ofloxacin are the most effective antibiotics for treating brucellosis. resistance to tigecycline is increasing, and we recommend that it be used in a combination regimen.
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Journal title:
iranian journal of pathologyجلد ۱۱، شماره ۳، صفحات ۲۳۸-۲۴۷
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